7P99

Structure of human USPL1 in complex with SUMO2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9762
Spacegroup name	P 1 21 1
Unit cell lengths	50.709, 69.884, 53.643
Unit cell angles	90.00, 90.66, 90.00

Refinement procedure

Resolution	41.040 - 1.800
R-factor	0.1756
Rwork	0.174
R-free	0.19740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	ALPHAFOLD
Data reduction software	AutoProcess
Data scaling software	AutoProcess
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.041	1.830
High resolution limit [Å]	1.799	1.799
Number of reflections	33606	1664
<I/σ(I)>	7.8
Completeness [%]	96.5
Redundancy	2.5
CC(1/2)	0.993	0.978

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		311	0.2M Potassium thiocyanate, 0.1M Sodium Acetate pH 5.0, 8% PEG20000, 8% PEG500MME