7P72

The PDZ domain of SNX27 complexed with the PDZ-binding motif of MERS-E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-17
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	61.170, 83.950, 106.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.994 - 2.150
R-factor	0.2011
Rwork	0.199
R-free	0.23970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5n7d 6sak
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.210
High resolution limit [Å]	2.150	2.150
Rmeas	0.252	2.260
Number of reflections	30598	2226
<I/σ(I)>	8.97	1.34
Completeness [%]	99.9	100
Redundancy	13.2	13.5
CC(1/2)	0.998	0.719

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	10% polyethylene glycol 8000, 100 mM imidazole buffered at pH 8.0