7P70

The PDZ-domain of SNTB1 complexed with the PDZ-binding motif of HPV35-E6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-17
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	54.800, 60.580, 140.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.897 - 2.000
R-factor	0.184
Rwork	0.183
R-free	0.20870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5n7d 2vrf
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.000	2.050
High resolution limit [Å]	2.000	2.000
Rmeas	0.153	2.130
Number of reflections	32358	2327
<I/σ(I)>	12.57	1.36
Completeness [%]	99.5
Redundancy	13.2
CC(1/2)	0.999	0.503

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% polyethylene glycol 3350, 200 mM sodium malonate buffered at pH 7.0