7P5D

Variant Surface Glycoprotein 3 (VSG3, MiTat1.3, VSG224) mutant (serine 317 and 319 to alanine)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-27
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0
Spacegroup name	I 21 3
Unit cell lengths	129.183, 129.183, 129.183
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.850 - 1.420
R-factor	0.1751
Rwork	0.174
R-free	0.19280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6elc
RMSD bond length	0.006
RMSD bond angle	0.967
Data reduction software	xia2
Data scaling software	pointless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.850	1.474
High resolution limit [Å]	1.420	1.420
Rmerge	0.116	2.767
Rmeas	0.119	2.843
Rpim	0.027	0.649
Number of reflections	66947	6654
<I/σ(I)>	20.08	1.19
Completeness [%]	100.0	99.64
Redundancy	20	19.1
CC(1/2)	1.000	0.455

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	25% PEG 3350, 300mM NaCl, 100mM HEPES, pH 7.5