7P5A

Variant Surface Glycoprotein 3 (VSG3, MiTat1.3, VSG224) mutant (serine 317 to alanine), single O-linked glycosylated at Ser319

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-29
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0
Spacegroup name	I 21 3
Unit cell lengths	129.396, 129.396, 129.396
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.750 - 1.950
R-factor	0.1832
Rwork	0.182
R-free	0.21320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6elc
RMSD bond length	0.004
RMSD bond angle	0.713
Data reduction software	xia2
Data scaling software	pointless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.750	2.020
High resolution limit [Å]	1.950	1.950
Rmerge	0.118	0.839
Rmeas	0.120	0.931
Rpim	0.021	0.388
Number of reflections	26355	2584
<I/σ(I)>	32.36	1.67
Completeness [%]	99.9	99.12
Redundancy	30.8	5.2
CC(1/2)	0.999	0.614

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	21% PEG 3350, 250mM NaCl, 100mM Tris, pH 8.2