7P41

Crystal Structure of human mARC1 A165T Variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976200
Spacegroup name	P 21 21 21
Unit cell lengths	61.063, 74.887, 111.164
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.920 - 1.600
R-factor	0.1613
Rwork	0.160
R-free	0.18630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fw2
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	53.520	53.520	1.630
High resolution limit [Å]	1.600	8.760	1.600
Rmerge	0.092	0.089	0.239
Rmeas	0.096	0.094	0.248
Rpim	0.026	0.027	0.067
Total number of observations	910173	5269	44210
Number of reflections	66911	438	3234
<I/σ(I)>	21	28	9.1
Completeness [%]	98.6	93.5	97.3
Redundancy	13.6	12	13.7
CC(1/2)	0.997	0.993	0.986

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	291	100 mM bis-tris-propane, 5 mM sodium molybdate, 27.5 % PEG3350