7P2R

Crystal structure of mouse PRMT6 in complex with inhibitor EML980

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-X
Temperature [K]	140
Detector technology	PIXEL
Collection date	2019-11-07
Detector	DECTRIS EIGER R 4M
Wavelength(s)	1.54178
Spacegroup name	P 1 21 1
Unit cell lengths	41.797, 118.687, 72.052
Unit cell angles	90.00, 102.79, 90.00

Refinement procedure

Resolution	45.340 - 2.300
R-factor	0.2024
Rwork	0.199
R-free	0.26850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6SQ6
Data reduction software	DIALS (3.3.3)
Data scaling software	DIALS (3.3.3)
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.340	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.114	0.513
Rmeas	0.131	0.612
Rpim	0.063	0.328
Number of reflections	30031	2717
<I/σ(I)>	9.24	1.83
Completeness [%]	98.6	89.91
Redundancy	4	3
CC(1/2)	0.991	0.782

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	100 mM Tris-HCl pH 7.5, 200 mM CHOONa, 19 PEG 3,350