7P2R
Crystal structure of mouse PRMT6 in complex with inhibitor EML980
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 140 |
| Detector technology | PIXEL |
| Collection date | 2019-11-07 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.797, 118.687, 72.052 |
| Unit cell angles | 90.00, 102.79, 90.00 |
Refinement procedure
| Resolution | 45.340 - 2.300 |
| R-factor | 0.2024 |
| Rwork | 0.199 |
| R-free | 0.26850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6SQ6 |
| Data reduction software | DIALS (3.3.3) |
| Data scaling software | DIALS (3.3.3) |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.340 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.114 | 0.513 |
| Rmeas | 0.131 | 0.612 |
| Rpim | 0.063 | 0.328 |
| Number of reflections | 30031 | 2717 |
| <I/σ(I)> | 9.24 | 1.83 |
| Completeness [%] | 98.6 | 89.91 |
| Redundancy | 4 | 3 |
| CC(1/2) | 0.991 | 0.782 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100 mM Tris-HCl pH 7.5, 200 mM CHOONa, 19 PEG 3,350 |
