7P2B
Crystal structure of human gelsolin amyloid mutant A551P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 105 |
| Detector technology | PIXEL |
| Collection date | 2020-02-10 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 170.070, 170.070, 152.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.910 - 3.000 |
| R-factor | 0.2154 |
| Rwork | 0.213 |
| R-free | 0.26250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ffn |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.910 | 3.080 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 44997 | 3291 |
| <I/σ(I)> | 11.53 | |
| Completeness [%] | 100.0 | |
| Redundancy | 27.3 | |
| CC(1/2) | 1.000 | 0.580 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 15% glycerol, 2.0 M ammonium sulfate, 0.1M tris HCl, pH8.5 |
