7P0E

Crystal structure of human filamin C domains 14-15 cardiovascular disease causing mutation G1676R

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-18
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.9655
Spacegroup name	P 21 21 21
Unit cell lengths	39.150, 59.460, 180.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.260 - 1.600
R-factor	0.1881
Rwork	0.187
R-free	0.22090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ouu
RMSD bond length	0.015
RMSD bond angle	1.234
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.260	1.657
High resolution limit [Å]	1.600	1.600
Rmerge	0.064
Rmeas	0.073	2.508
Rpim	0.035	1.166
Number of reflections	53624	4752
<I/σ(I)>	9.97	0.5
Completeness [%]	94.0	79.64
Redundancy	4
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	295.15	0.1 M BIS-TRIS pH 6.0, 26.3 % w/v PEG 3350, 5% w/v Glycerol