7OZB
FGFR1 kinase domain (residues 458-765) with mutations C488A, C584S in complex with 38.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9159 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 206.260, 57.560, 65.790 |
| Unit cell angles | 90.00, 107.22, 90.00 |
Refinement procedure
| Resolution | 98.507 - 1.710 |
| Rwork | 0.209 |
| R-free | 0.24420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5a4c |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.383 |
| Data reduction software | xia2 |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 98.510 | 1.750 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Rmerge | 0.042 | 0.969 |
| Rmeas | 0.049 | |
| Rpim | 0.026 | 0.737 |
| Number of reflections | 77609 | 5672 |
| <I/σ(I)> | 14.1 | 1.3 |
| Completeness [%] | 97.4 | 96.8 |
| Redundancy | 3.6 | 3.7 |
| CC(1/2) | 0.998 | 0.707 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 291 | 0.185M ammonium sulfate, 20% v/v ethylene glycol, 17% w/v PEG 8000, 0.1M PCPT |
