7OXN

Crystal Structure of TAP01 in complex with cyclised amyloid beta peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97949
Spacegroup name	I 1 2 1
Unit cell lengths	80.831, 47.494, 124.427
Unit cell angles	90.00, 90.95, 90.00

Refinement procedure

Resolution	67.260 - 2.500
Rwork	0.228
R-free	0.26500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ow1
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.360	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.036
Number of reflections	16607	1619
<I/σ(I)>	11.9
Completeness [%]	100.0
Redundancy	1.9
CC(1/2)	0.996	0.976

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	292	20% PEG 6K 0.1 M HEPES, pH 7.0 0.01 M zinc chloride