7OX5
hIL-9:hIL-9Ra complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-19 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.450, 107.810, 159.550 |
| Unit cell angles | 90.00, 90.77, 90.00 |
Refinement procedure
| Resolution | 47.690 - 3.090 |
| R-factor | 0.2228 |
| Rwork | 0.220 |
| R-free | 0.27960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b5i |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.690 | 3.270 |
| High resolution limit [Å] | 3.090 | 3.090 |
| Number of reflections | 52814 | 8141 |
| <I/σ(I)> | 11.39 | |
| Completeness [%] | 98.9 | |
| Redundancy | 3.5 | |
| CC(1/2) | 0.998 | 0.281 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.1 M ammonium sulfate, 0.1 M TRIS, pH 5.6, 18% PEG 1500 |
