7OWZ

Heterodimeric murine tRNA-guanine transglycosylase in complex with queuine and in the presence of Anderson-Evans type (TEW) and Strandberg type polyoxometalate (POM)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.918400
Spacegroup name	P 62 2 2
Unit cell lengths	199.500, 199.500, 90.586
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.600 - 2.600
R-factor	0.2151
Rwork	0.214
R-free	0.23940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6h62 6fv5
RMSD bond length	0.003
RMSD bond angle	1.010
Data reduction software	XDS (1.02)
Data scaling software	XDS (1.02)
Phasing software	PHASER (7.0.047)
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.600	2.750
High resolution limit [Å]	2.600	2.600
Number of reflections	62234	9974
<I/σ(I)>	24.06
Completeness [%]	99.9
Redundancy	12.75
CC(1/2)	1.000	0.833

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	100 mM Na/K phosphate pH 6.8, 200 mM Sodium chloride, 14 % (w/v) PEG 1000, 1 mM TEW