7OPR

Rab27a fusion with Slp2a-RBDa1 effector covalent adduct with CB1 in C123

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	61.385, 76.663, 118.243
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.330 - 2.320
R-factor	0.1862
Rwork	0.183
R-free	0.25490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bc1
Data reduction software	DIALS
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.330	2.403
High resolution limit [Å]	2.320	2.320
Rmerge	0.059	0.325
Rmeas	0.083	0.460
Rpim	0.059	0.325
Number of reflections	49537	4902
<I/σ(I)>	9.77	2.23
Completeness [%]	99.8	99.67
Redundancy	2	2
CC(1/2)	0.993	0.832

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277.15	0.15M Ammonium sulphate, 0.1M MES pH 6.0, 15% w/v PEG 4000