7OPB

IL7R in complex with an antagonist

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-21
Detector	DECTRIS PILATUS3 X 2M
Wavelength(s)	0.87313
Spacegroup name	P 32
Unit cell lengths	132.182, 132.182, 58.877
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.960 - 2.144
R-factor	0.1946
Rwork	0.194
R-free	0.21090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3di2
RMSD bond length	0.008
RMSD bond angle	0.970
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.960	2.270
High resolution limit [Å]	2.144	2.144
Rmeas	0.204	1.940
Rpim	0.080	0.868
Number of reflections	62839	9978
<I/σ(I)>	6.92	1.04
Completeness [%]	99.6	97.6
Redundancy	8.19
CC(1/2)	0.993	0.442

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293.15	0,1 M Phosphate/Citrate, 25% v/v PEG Smear Low Cryoprotected with 25% PEG-400