7OOT
X-ray Structure of Interferon Regulatory Factor 4 DNA binding domain bound to an interferon-stimulated response element
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-08-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9688 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.780, 83.250, 88.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.270 - 2.250 |
| R-factor | 0.2038 |
| Rwork | 0.202 |
| R-free | 0.23830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7o56 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.042 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.300 | 2.290 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.112 | 1.136 |
| Number of reflections | 23181 | 1086 |
| <I/σ(I)> | 11.1 | 1.1 |
| Completeness [%] | 99.3 | |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.1M Tris HCl pH 8.5, 30% PEG 4000, 0.2M lithium sulfate |
