7OMF
Structure of a minimal SF3B core in complex with sudemycin D6 (form I)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-08-29 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 106.355, 109.846, 253.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.030 | 2.970 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.086 | 4.094 |
Rmeas | 0.090 | 4.249 |
Rpim | 0.024 | 1.131 |
Number of reflections | 66573 | 4382 |
<I/σ(I)> | 19.1 | 0.7 |
Completeness [%] | 99.9 | 98.9 |
Redundancy | 13.7 | 13.8 |
CC(1/2) | 1.000 | 0.389 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 0.1M Bis-tris propane pH 7.4, 0.2M KSCN, 12% (w/v) PEG-3350, 10%(v/v) Glycerol |