7OD9

Crystal structure of activated CheY fused to the C-terminal domain of CheF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97625
Spacegroup name	P 1 21 1
Unit cell lengths	56.290, 70.870, 59.820
Unit cell angles	90.00, 91.60, 90.00

Refinement procedure

Resolution	44.070 - 2.300
R-factor	0.2524
Rwork	0.249
R-free	0.30660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ekh
RMSD bond length	0.005
RMSD bond angle	0.864
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.070	2.383
High resolution limit [Å]	2.300	2.300
Number of reflections	20987	2097
<I/σ(I)>	7.66	0.88
Completeness [%]	99.6
Redundancy	6.5
CC(1/2)	0.997	0.577

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.2 MgCl2, 0.1 M Tris pH 8.5, 20 % (w/v) PEG 8000