7O4G

Human phosphomannomutase 2 (PMM2) wild-type soaked with the activator glucose 1,6-bisphosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97926
Spacegroup name	P 65 2 2
Unit cell lengths	71.144, 71.144, 361.266
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	60.210 - 2.660
R-factor	0.2261
Rwork	0.224
R-free	0.27300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7o0c
RMSD bond length	0.009
RMSD bond angle	1.033
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (v1.19)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	361.270	2.790
High resolution limit [Å]	2.660	2.660
Rmerge	0.417	3.011
Rpim	0.085	0.813
Number of reflections	16779	2150
<I/σ(I)>	9.2	1.8
Completeness [%]	100.0	100
Redundancy	25.3	26.7
CC(1/2)	0.996	0.625

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	Drops made by mixing 1 microliter of protein solution and 1 microliter of crystallization deposit solution. Protein solution contained 5 mg/ml protein in 20 mM Hepes pH 7.5 and 0.2 M NaCl. Crystallization solution contained 0.3-0.4 M MgCl2, 24% PEG3350 and 0.1 M HEPES pH 7.5. Crystals were soaked for 5-10 min in mother liquor with 6 mM glucose 1,6-bisphophate.