7O2X

Crystal structure of the human METTL3-METTL14 complex with compound T180

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-27
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 32 2 1
Unit cell lengths	63.950, 63.950, 225.720
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.720 - 2.800
R-factor	0.2112
Rwork	0.208
R-free	0.27410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7acd
RMSD bond length	0.010
RMSD bond angle	1.091
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.800	2.970
High resolution limit [Å]	2.800	2.800
Number of reflections	13970	2173
<I/σ(I)>	7.15
Completeness [%]	99.8
Redundancy	7.9
CC(1/2)	0.988	0.563

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	20% PEG 3350, 400mM Mg acetate