7NXE

Structure of the Phospholipase C gamma 1 tSH2 domain in complex with a phosphorylated KSHV pK15 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-19
Detector	DECTRIS PILATUS 12M
Wavelength(s)	0.976
Spacegroup name	P 21 21 2
Unit cell lengths	54.660, 77.030, 59.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.580 - 2.100
R-factor	0.2526
Rwork	0.250
R-free	0.31380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fbn
RMSD bond length	0.008
RMSD bond angle	0.920
Data reduction software	XDS
Data scaling software	pointless
Phasing software	PHASER
Refinement software	BUSTER (2.10.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.580	2.220
High resolution limit [Å]	2.100	2.100
Rmeas	0.085	0.809
Number of reflections	15318	15318
<I/σ(I)>	12.19
Completeness [%]	99.5	97.8
Redundancy	7.07	7.25
CC(1/2)	0.998	0.895

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	30% PEG 4000, 0.1M TrisHCl pH 8.5, 0.2M sodium acetate