7NWU

Co-crystal structure of UPF3B-RRM-NOPS-L with UPF2-MIF4GIII

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	277
Detector technology	PIXEL
Collection date	2020-06-26
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 41 2 2
Unit cell lengths	130.593, 130.593, 267.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.600
R-factor	0.2187
Rwork	0.217
R-free	0.25590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uw4
RMSD bond length	0.004
RMSD bond angle	0.536
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2-4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.550
High resolution limit [Å]	1.309	12.750	2.500
Rpim	0.061	0.010	0.809
Number of reflections	80565	678	4529
<I/σ(I)>	10.2	39.1	1
Completeness [%]	99.9	92.6	100
Redundancy	14.2	14.1	14.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293.15	0.1M Ca acetate 0.1M MES 15% PEG 400 P 41 2 2