7NUU

Crystal structure of human AMDHD2 in complex with Zn

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-09-15
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	61.777, 84.336, 154.166
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	77.083 - 1.836
R-factor	0.183
Rwork	0.182
R-free	0.20560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7nut
Data reduction software	XDS (Mar 15, 2019)
Data scaling software	XDS (Mar 15, 2019)
Phasing software	PHENIX (1.16_3549)
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.083	1.902
High resolution limit [Å]	1.836	1.836
Number of reflections	71036	6953
<I/σ(I)>	12.53
Completeness [%]	99.9
Redundancy	6.6
CC(1/2)	0.999	0.499

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1 M Bis tris propane pH 8.25, 0.25 M Sodium nitrate, 20 % PEG3350