7NUT

Crystal structure of human AMDHD2 in complex with Zn and GlcN6P

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-06-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	63.261, 161.449, 86.621
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.707 - 1.898
R-factor	0.1857
Rwork	0.185
R-free	0.21270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vhl
Data reduction software	XDS (Mar 15, 2019)
Data scaling software	XDS (Mar 15, 2019)
Phasing software	PHENIX (1.16_3549)
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.707	1.970
High resolution limit [Å]	1.898	1.898
Number of reflections	70760	6907
<I/σ(I)>	11.46
Completeness [%]	99.7
Redundancy	6.1
CC(1/2)	0.999	0.494

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1 M Bis tris propane pH 8.25, 0.25 M Sodium nitrate, 20 % PEG3350