7NOX

Structure of SGBP BO2743 from Bacteroides ovatus in complex with mixed-linked gluco-nonasaccharide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-26
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	87.842, 88.437, 156.148
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.860 - 1.430
Rwork	0.162
R-free	0.18970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5j5u
Data reduction software	STARANISO
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.19)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.320	1.550
High resolution limit [Å]	1.430	1.430
Number of reflections	175289	8764
<I/σ(I)>	1.6
Completeness [%]	96.5
Redundancy	9
CC(1/2)	1.000	0.740

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Protein at 8 mg/mL; 25% PEG 3350, 0.1 M Bis-Tris pH 5.5, 0.2 mM MgCl2.6H2O