7NOT
Crystal structure of Mycobacterium tuberculosis ArgC in complex with nicotinamide adenine dinucleotide phosphate (NADP+) and 5-Methoxy-3-indoleacetic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-30 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 83.400, 132.000, 124.550 |
Unit cell angles | 90.00, 97.06, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 63.910 | 63.910 | 2.610 |
High resolution limit [Å] | 2.540 | 11.360 | 2.540 |
Rmerge | 0.235 | 0.055 | 2.353 |
Rmeas | 0.260 | 0.062 | 2.598 |
Rpim | 0.110 | 0.027 | 1.082 |
Total number of observations | 463192 | 5190 | 36392 |
Number of reflections | 87548 | 993 | 6486 |
<I/σ(I)> | 7.3 | 26.1 | 0.8 |
Completeness [%] | 99.3 | 96.6 | 99.9 |
Redundancy | 5.3 | 5.2 | 5.6 |
CC(1/2) | 0.986 | 0.990 | 0.317 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M Bis-Tris pH 7 17% PEG Smear High 70 mM phosphate/citrate pH 5.6 20% glycerol |