7NMZ
Structure of 14-3-3 eta in complex with Nedd4-2(335-455) containing two 14-3-3 binding motifs Ser342 and Ser448
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | LIQUID ANODE |
| Source details | Excillum MetalJet D2+ 70 kV |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-01-20 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.3418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.860, 58.950, 106.760 |
| Unit cell angles | 90.00, 90.69, 90.00 |
Refinement procedure
| Resolution | 32.690 - 2.303 |
| R-factor | 0.2012 |
| Rwork | 0.199 |
| R-free | 0.23460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c63 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.610 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.690 | 2.385 |
| High resolution limit [Å] | 2.303 | 2.303 |
| Rmeas | 0.064 | 0.655 |
| Number of reflections | 32703 | 3215 |
| <I/σ(I)> | 19.68 | 1.94 |
| Completeness [%] | 99.9 | |
| Redundancy | 4.73 | 3.99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.15 | 12.5% w/v PEG 1000, 12.5% w/v PEG 3350, 12.5% v/v MPD, 0.03M of each NPS (sodium nitrate, sodium phosphate dibasic, ammonium sulfate), 0.1Mbicine/Trizma base pH 8.5, 30% sacharose |
