7NMU
Crystal structure of human platelet glycoprotein VI in complex with an inhibitory nanobody.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 283.15 |
| Detector technology | PIXEL |
| Collection date | 2019-10-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.96864 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.906, 84.751, 124.041 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.080 - 2.500 |
| Rwork | 0.174 |
| R-free | 0.23510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gi7 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.551 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.750 | 2.560 |
| High resolution limit [Å] | 2.498 | 2.500 |
| Rmerge | 0.122 | |
| Number of reflections | 26173 | 11961 |
| <I/σ(I)> | 8.67 | |
| Completeness [%] | 98.6 | |
| Redundancy | 6.3 | |
| CC(1/2) | 0.991 | 0.834 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 283.15 | 0.2 M calcium acetate, 0.1M sodium cacodylate pH 6.5, 18% PEG8K |
