7NL7
Crystal Structure of DC-SIGN in complex with a triazole-based glycomimetic ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00001 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 59.346, 59.346, 75.180 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 51.390 - 2.100 |
| R-factor | 0.1938 |
| Rwork | 0.193 |
| R-free | 0.21780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sl4 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.1_3865) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.390 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.408 | 2.350 |
| Rpim | 0.094 | 0.530 |
| Number of reflections | 9333 | 906 |
| <I/σ(I)> | 5.1 | 1.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 19.5 | 20.2 |
| CC(1/2) | 0.987 | 0.745 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M MgCl2, 0.1M Hepes 7.5, 10% PEG4000 |
