7NJC

Crystal structure of the Toxoplasma CPSF4 YTH-domain in complex with a 7 mer m6A-modified RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-13
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9795
Spacegroup name	P 1
Unit cell lengths	32.442, 35.153, 38.350
Unit cell angles	114.68, 100.70, 97.65

Refinement procedure

Resolution	31.040 - 1.380
R-factor	0.1456
Rwork	0.143
R-free	0.16850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4r3i
RMSD bond length	0.006
RMSD bond angle	0.915
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.040	1.450
High resolution limit [Å]	1.380	1.380
Number of reflections	19180	288
<I/σ(I)>	17.3	3.1
Completeness [%]	62.9	6.5
Redundancy	3.4	1.8
CC(1/2)	0.999	0.989

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M sodium acetate pH: 4.6 in 40% PEG 200