7NIY
E. coli NfsA with FMN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-18 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.934 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 91.911, 52.003, 64.695 |
Unit cell angles | 90.00, 133.78, 90.00 |
Refinement procedure
Resolution | 32.360 - 1.030 |
R-factor | 0.1056 |
Rwork | 0.105 |
R-free | 0.11830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fv5 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.752 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.360 | 1.056 |
High resolution limit [Å] | 1.030 | 1.030 |
Number of reflections | 99892 | 7069 |
<I/σ(I)> | 27.89 | |
Completeness [%] | 96.7 | 92.3 |
Redundancy | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 30 % PEG 3,350; 100 mM Imidazole, 20 mM Hydroquinone, 15 % Ethylene Glycol |