7NE0
Structure of the ternary complex between Netrin-1, Repulsive-Guidance Molecule-B (RGMB) and Neogenin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-07 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97630 |
Spacegroup name | H 3 2 |
Unit cell lengths | 136.780, 136.780, 430.110 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 79.620 | 3.310 |
High resolution limit [Å] | 3.250 | 3.250 |
Rmerge | 0.167 | |
Rpim | 0.046 | |
Number of reflections | 24856 | 1184 |
<I/σ(I)> | 16.7 | 1.2 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 8.1 | |
CC(1/2) | 0.999 | 0.321 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298.15 | 0.1 M imidazole/MES pH 6.5, 10% (w/v) PEG 8000, 20% (v/v) ethylene glycol, 30 mM sodium nitrate, 30 mM sodium phosphate, 30 mM ammonium sulphate |