7NCX
Crystal structure of GH30 (double mutant EE) from Thermothelomyces thermophila.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-07 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.887, 87.315, 107.917 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.696 - 1.360 |
| Rwork | 0.122 |
| R-free | 0.15810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6krl |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.576 |
| Data reduction software | Aimless |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.900 | 1.380 |
| High resolution limit [Å] | 1.360 | 1.360 |
| Number of reflections | 79578 | 3715 |
| <I/σ(I)> | 19.8 | |
| Completeness [%] | 99.7 | |
| Redundancy | 12.1 | |
| CC(1/2) | 1.000 | 0.539 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG1500, TBG buffer |
