7NBI

Crystal structure of a monomeric FLT3 Ligand variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97625
Spacegroup name	P 1
Unit cell lengths	28.300, 43.490, 46.360
Unit cell angles	82.82, 85.41, 85.10

Refinement procedure

Resolution	18.420 - 1.650
R-factor	0.1662
Rwork	0.164
R-free	0.20260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ete
RMSD bond length	0.018
RMSD bond angle	1.490
Data reduction software	XDS (VERSION Jan 31, 2020)
Data scaling software	XDS (VERSION Jan 31, 2020)
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.640	1.709
High resolution limit [Å]	1.650	1.650
Rmeas	0.077	0.567
Number of reflections	70278	4053
<I/σ(I)>	10.6	2.33
Completeness [%]	94.9	89.2
Redundancy	8.81	2.37
CC(1/2)	0.996	0.728

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	2.0 M ammonium sulfate, 0.1 M HEPES