7N96

Crystal structure of D103A human Galectin-7 mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-02-19
Detector	RAYONIX MX300HE
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	53.510, 67.110, 68.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.770 - 2.380
R-factor	0.2221
Rwork	0.218
R-free	0.25940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bkz
RMSD bond length	0.005
RMSD bond angle	0.882
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.7.12)
Refinement software	PHENIX (1.19.2-4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.770	47.780	2.470
High resolution limit [Å]	2.380	5.130	2.380
Rmerge	0.154	0.046	1.520
Rmeas	0.163	0.048	1.603
Rpim	0.052	0.016	0.502
Number of reflections	10282	1111	1003
<I/σ(I)>	13.7	35.8	1.9
Completeness [%]	100.0	100	99.8
Redundancy	9.6	8.8	9.8
CC(1/2)	0.998	0.999	0.719

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	295.15	0.1 M NaCl, 0.1 M Tris pH 8, 20 % PEG 6000 ,15% Glycerol