7N93
P70 S6K1 IN COMPLEX WITH MSC2363318A-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-02-06 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.020, 75.450, 151.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.740 |
| R-factor | 0.2155 |
| Rwork | 0.211 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a60 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.208 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.530 | 2.890 |
| High resolution limit [Å] | 2.739 | 2.739 |
| Rmerge | 0.089 | 0.545 |
| Rmeas | 0.076 | 0.603 |
| Number of reflections | 18874 | 2854 |
| <I/σ(I)> | 16.64 | 2.7 |
| Completeness [%] | 98.9 | 93.5 |
| Redundancy | 6.27 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 0.2M Bis-Tris pH 5.5, 0.1M Li2SO4, 26-28% PEG3350 |
