7N8S

LINE-1 endonuclease domain complex with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2019-11-19
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 61 2 2
Unit cell lengths	91.613, 91.613, 229.822
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.740 - 2.790
R-factor	0.2693
Rwork	0.264
R-free	0.32180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vyb
RMSD bond length	0.006
RMSD bond angle	0.818
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.850
High resolution limit [Å]	2.790	2.800
Rmerge	0.139	1.565
Rmeas	0.148	1.660
Rpim	0.050	0.548
Number of reflections	14939	728
<I/σ(I)>	18.5	1.8
Completeness [%]	100.0	100
Redundancy	8.6	9
CC(1/2)	0.998	0.675

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.5	298	0.2M NH4 CH3 CO2; 15% PEG 4000