7N8K
LINE-1 endonuclease domain complex with Mg
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 180 |
Detector technology | IMAGE PLATE |
Collection date | 2019-07-18 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.857, 126.776, 44.854 |
Unit cell angles | 90.00, 98.94, 90.00 |
Refinement procedure
Resolution | 33.300 - 2.010 |
R-factor | 0.1949 |
Rwork | 0.192 |
R-free | 0.23610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vyb |
RMSD bond length | 0.008 |
RMSD bond angle | 1.099 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.040 |
High resolution limit [Å] | 2.010 | 2.010 |
Rmerge | 0.089 | 0.750 |
Rmeas | 0.104 | |
Rpim | 0.055 | |
Number of reflections | 30980 | 1505 |
<I/σ(I)> | 16.3 | 1.54 |
Completeness [%] | 98.2 | 93.7 |
Redundancy | 3.5 | 3 |
CC(1/2) | 0.998 | 0.627 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6 | 298 | 0.1M LiSO4; 26% PEG 2000 MME |