7N8E

PptT PAP(CoA) 9056 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	120
Detector technology	PIXEL
Collection date	2017-12-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97949
Spacegroup name	C 1 2 1
Unit cell lengths	134.692, 63.283, 81.228
Unit cell angles	90.00, 124.27, 90.00

Refinement procedure

Resolution	67.120 - 1.740
R-factor	0.2063
Rwork	0.205
R-free	0.24190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ct5
RMSD bond length	0.007
RMSD bond angle	0.778
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.120	1.802
High resolution limit [Å]	1.740	1.740
Number of reflections	57883	5764
<I/σ(I)>	9.5
Completeness [%]	99.7
Redundancy	2
CC(1/2)	0.996	0.808

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	291.15	0.1 M Bis-Tris Propane pH 6.7, 1.5 M Magnesium Sulfate with protein: condition 1:1