7N3Y
Crystal Structure of Saccharomyces cerevisiae Apn2 Catalytic Domain E59Q/D222N Mutant in Complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2015-07-24 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.408, 101.473, 144.973 |
| Unit cell angles | 90.00, 90.14, 90.00 |
Refinement procedure
| Resolution | 36.970 - 2.730 |
| R-factor | 0.201 |
| Rwork | 0.200 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3g0r |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.610 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.970 | 2.830 |
| High resolution limit [Å] | 2.730 | 2.730 |
| Rmeas | 0.085 | 0.891 |
| Number of reflections | 51375 | 4598 |
| <I/σ(I)> | 18.4 | 1.44 |
| Completeness [%] | 96.8 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M sodium citrate, 20% PEG 3350 |
