7MU8
Structure of the minimally glycosylated human CEACAM1 N-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 93.455, 93.455, 134.665 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.700 - 1.700 |
| R-factor | 0.2025 |
| Rwork | 0.202 |
| R-free | 0.21720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4qxw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.023 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.730 | 1.554 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.072 | 2.009 |
| Rmeas | 0.076 | 2.103 |
| Rpim | 0.022 | 0.618 |
| Number of reflections | 25094 | 3588 |
| <I/σ(I)> | 12.88 | 1.11 |
| Completeness [%] | 99.8 | 99.89 |
| Redundancy | 11.2 | 11.5 |
| CC(1/2) | 0.999 | 0.494 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 100 mM MES, pH 6.5, 2M Ammonium sulfate |
