7MQY

Zebrafish CNTN4 APLP2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2019-10-19
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	61.660, 44.980, 99.829
Unit cell angles	90.00, 95.84, 90.00

Refinement procedure

Resolution	49.960 - 2.460
R-factor	0.1923
Rwork	0.189
R-free	0.22090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5e4s 3ktm
RMSD bond length	0.004
RMSD bond angle	0.676
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.310	2.560
High resolution limit [Å]	2.460	2.460
Rmerge	0.090	0.597
Rmeas	0.096	0.646
Rpim	0.035	0.243
Number of reflections	20199	2266
<I/σ(I)>	15.6	3.9
Completeness [%]	100.0	99.9
Redundancy	7.4	7
CC(1/2)	0.998	0.909

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM Na-acetate pH 5.2, 30% (w/v) PEG 8,000