7MO2

Crystal Structure of the ZnF2 of Nucleoporin NUP153 in complex with Ran-GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03317
Spacegroup name	P 1 21 1
Unit cell lengths	58.089, 60.381, 79.426
Unit cell angles	90.00, 90.08, 90.00

Refinement procedure

Resolution	29.040 - 1.650
R-factor	0.2125
Rwork	0.211
R-free	0.24100
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	AutoSol
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.040	29.040	1.680
High resolution limit [Å]	1.650	9.030	1.650
Rmerge	0.073	0.032	1.079
Rmeas	0.079	0.035	1.186
Rpim	0.030	0.013	0.481
Total number of observations		2970	18540
Number of reflections	127956	426	3167
<I/σ(I)>	13.8	41.6	1.5
Completeness [%]	98.5	97.6	96.6
Redundancy	6.8	7	5.9
CC(1/2)	0.999	0.998	0.493

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	294	19% w/v PEG3350, 0.1 M Bis-Tris