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7MO1

Crystal Structure of the ZnF1 of Nucleoporin NUP153 in complex with Ran-GDP

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSSRL BEAMLINE BL12-2
Synchrotron siteSSRL
BeamlineBL12-2
Temperature [K]100
Detector technologyPIXEL
Collection date2020-10-19
DetectorDECTRIS PILATUS 6M
Wavelength(s)1.03317
Spacegroup nameP 21 21 2
Unit cell lengths60.276, 79.619, 55.063
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution28.440 - 1.600
R-factor0.1523
Rwork0.151
R-free0.17540
Structure solution methodSAD
Data reduction softwareXDS
Data scaling softwareAimless (0.7.4)
Phasing softwareAutoSol
Refinement softwarePHENIX (1.18.2)
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]28.44028.4401.630
High resolution limit [Å]1.6008.7501.600
Rmerge0.0780.0321.717
Rmeas0.0810.0331.790
Rpim0.0220.0090.498
Total number of observations303222144
Number of reflections678162651762
<I/σ(I)>1968.71.5
Completeness [%]100.097.899.5
Redundancy1311.412.6
CC(1/2)1.0000.9990.653
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP629422% w/v PEG3350, 0.1 M Bis-Tris

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