7MO1
Crystal Structure of the ZnF1 of Nucleoporin NUP153 in complex with Ran-GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.03317 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 60.276, 79.619, 55.063 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.440 - 1.600 |
R-factor | 0.1523 |
Rwork | 0.151 |
R-free | 0.17540 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | AutoSol |
Refinement software | PHENIX (1.18.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 28.440 | 28.440 | 1.630 |
High resolution limit [Å] | 1.600 | 8.750 | 1.600 |
Rmerge | 0.078 | 0.032 | 1.717 |
Rmeas | 0.081 | 0.033 | 1.790 |
Rpim | 0.022 | 0.009 | 0.498 |
Total number of observations | 3032 | 22144 | |
Number of reflections | 67816 | 265 | 1762 |
<I/σ(I)> | 19 | 68.7 | 1.5 |
Completeness [%] | 100.0 | 97.8 | 99.5 |
Redundancy | 13 | 11.4 | 12.6 |
CC(1/2) | 1.000 | 0.999 | 0.653 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 294 | 22% w/v PEG3350, 0.1 M Bis-Tris |