7MO0

Crystal Structure of Nucleoporin NUP50 Ran-Binding Domain in Complex with Ran-GPPNHP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97942
Spacegroup name	P 21 21 21
Unit cell lengths	66.590, 73.170, 152.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.730 - 2.450
R-factor	0.2134
Rwork	0.212
R-free	0.23870
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	xia2
Phasing software	AutoSol
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.730	29.730	2.540
High resolution limit [Å]	2.450	5.270	2.450
Rmeas	0.125	0.058	2.693
Rpim	0.034	0.016	0.733
Total number of observations		37155	36629
Number of reflections	52982	3022	2762
<I/σ(I)>	15.3	40.6	1.1
Completeness [%]	100.0	100	100
Redundancy	13.1	12.3	13.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	294	25% w/v PEG3350, 0.2 M ammonium sulfate, 0.1 M HEPES