7MNU

Crystal Structure of the ZnF7 of Nucleoporin NUP358/RanBP2 in complex with Ran-GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.2828
Spacegroup name	P 65 2 2
Unit cell lengths	92.720, 92.720, 196.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.990 - 2.000
R-factor	0.2027
Rwork	0.202
R-free	0.22420
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	xia2
Phasing software	AutoSol
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.990	28.990	2.070
High resolution limit [Å]	2.000	4.300	2.000
Rmeas	0.166	0.063	2.345
Rpim	0.040	0.016	0.574
Total number of observations	584705	58924	55080
Number of reflections	34531	3770	3346
<I/σ(I)>	14	39.5	1.4
Completeness [%]	100.0	100	100
Redundancy	16.9	15.6	16.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	19% (w/v) PEG 3350; 0.1 M Bis-Tris