7MNM

Crystal structure of the N-terminal domain of NUP358/RanBP2 (residues 1-752) T585M mutant in complex with Fab fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	P 65 2 2
Unit cell lengths	160.408, 160.408, 642.167
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.860 - 4.700
R-factor	0.2062
Rwork	0.204
R-free	0.25380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ga0 5cws
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.860	29.860	5.020
High resolution limit [Å]	4.700	13.290	4.700
Rmerge	0.586	0.133	4.818
Rmeas	0.594	0.135	4.881
Rpim	0.094	0.023	0.778
Total number of observations	1037121	41203	182414
Number of reflections	26575	1244	4697
<I/σ(I)>	6.6	24.6	1.1
Completeness [%]	99.6	91.2	100
Redundancy	39	33.1	38.8
CC(1/2)	0.996	0.999	0.586

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	2.5 % (w/v) PEG 4,000; 0.15 M sodium acetate; 0.1 M sodium citrate