7MKK

Crystal structure of Drosophila Panoramix in complex with Sov NTD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 64 2 2
Unit cell lengths	127.389, 127.389, 96.012
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.829 - 2.500
R-factor	0.2282
Rwork	0.227
R-free	0.25950
Structure solution method	SAD
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.500	2.500
Number of reflections	16430	2329
<I/σ(I)>	22.8
Completeness [%]	100.0
Redundancy	12.6
CC(1/2)	0.999	0.930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M CHES pH 9.5, 30% (w/v) PEG 3000