7MFU
Crystal structure of synthetic nanobody (Sb14+Sb68) complexes with SARS-CoV-2 receptor binding domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.820, 83.050, 92.830 |
| Unit cell angles | 90.00, 106.71, 90.00 |
Refinement procedure
| Resolution | 40.270 - 1.700 |
| R-factor | 0.1828 |
| Rwork | 0.181 |
| R-free | 0.21490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7klw |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.734 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.270 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.086 | 0.765 |
| Rmeas | 0.104 | 0.925 |
| Rpim | 0.057 | 0.510 |
| Number of reflections | 105129 | 9993 |
| <I/σ(I)> | 8.87 | 1.71 |
| Completeness [%] | 98.4 | 93.8 |
| Redundancy | 3.1 | 3.1 |
| CC(1/2) | 0.995 | 0.640 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% PEG 8000, 0.1M Hepes pH 7.5 |
