7MFO
X-ray structure of the L136 Aminotransferase from Acanthamoeba polyphaga mimivirus in the presence of TDP and PMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-06-23 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9790 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.885, 126.875, 206.365 |
Unit cell angles | 90.00, 90.89, 90.00 |
Refinement procedure
Resolution | 33.780 - 1.700 |
R-factor | 0.1839 |
Rwork | 0.182 |
R-free | 0.22150 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.619 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 397722 | 18360 |
<I/σ(I)> | 36.2 | 5 |
Completeness [%] | 94.3 | 87.4 |
Redundancy | 4.1 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 7.5 | 293 | protein incubated with 1 mM PLP and 5 mM TDP-4-aminoquinovose. 11-14% PEG-8000, 200 mM KCl, 100 mM HEPES |